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10X Genomics cdna amplification
Cdna Amplification, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+amplification/pmc13243389-202-4-15
Average 86 stars, based on 1 article reviews
cdna amplification - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Reverse Transcription:

Article Title: Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLC
Article Snippet: .. Reverse transcription, second-strand synthesis, cDNA amplification, and spatial library construction followed the manufacturer’s official protocol (10x Genomics). .. Libraries were sequenced on Illumina NovaSeq 6000.

Article Title: Specification of the interstitial cell lineage in the fetal mouse ovary requires balanced Notch signaling
Article Snippet: Up to 16,000 live cells were loaded into the Single Cell Chip to generate single cell emulsion in Chromium Controller with Chromium Single Cell 3’ Library & Gel Bead Kit v3.1 (10x Genomics, 1000268). .. Reverse transcription of mRNA and cDNA amplification were carried out following the manufacture’s instruction (10x Genomics, 1000268). ..

Amplification:

Article Title: Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLC
Article Snippet: .. Reverse transcription, second-strand synthesis, cDNA amplification, and spatial library construction followed the manufacturer’s official protocol (10x Genomics). .. Libraries were sequenced on Illumina NovaSeq 6000.

Article Title: Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer.
Article Snippet: .. The spatial gene expression process, including tissue permeabilization, second strand synthesis and cDNA amplification, was performed according to the manufacturer’s instructions (10X Genomics; #CG000239). cDNA quality was next assessed using Agilent High sensitivity DNA Kit (Agilent, #5067-4626). .. The spatial gene libraries were constructed using Visium Spatial Library Construction Kit (10X Genomics, PN-1000184).

Article Title: E-cadherin inactivation shapes tumor microenvironment specificities in invasive lobular breast cancer.
Article Snippet: .. Spatial gene expression processing, including tissue permeabilization, second-strand synthesis, and cDNA amplification, was then performed according to the manufacturer’s instructions (10X Genomics; #CG000239), following the workflow previously reported in 33. cDNA quality was subsequently assessed using the Agilent High Sensitivity DNA Kit (Agilent, #5067-4626). .. Spatial gene libraries were generated using the Visium Spatial Library Construction Kit (10X Genomics, PN-1000184).

Article Title: Specification of the interstitial cell lineage in the fetal mouse ovary requires balanced Notch signaling
Article Snippet: Up to 16,000 live cells were loaded into the Single Cell Chip to generate single cell emulsion in Chromium Controller with Chromium Single Cell 3’ Library & Gel Bead Kit v3.1 (10x Genomics, 1000268). .. Reverse transcription of mRNA and cDNA amplification were carried out following the manufacture’s instruction (10x Genomics, 1000268). ..

Article Title: Automated high-throughput profiling of single-cell total transcriptome with scComplete-seq
Article Snippet: .. After in-droplet cDNA synthesis, the droplets were de-emulsified to recover cDNA, and cDNA amplification was performed according to the 10X Genomics Chromium protocol. ..

Article Title: The chromatin and single-cell transcriptional landscapes of CD4 T cells in inflammatory bowel disease link risk loci with a proinflammatory Th17 cell population.
Article Snippet: .. Approximately 1,000 cells of each biopsy were captured and encapsulated before cDNA amplification using the 10X Genomics Chromium Platform. .. Samples were prepared as outlined by 10X Genomics Single Cell 3’ Reagent Kits v2 user guide.

Article Title: A Machine Learning–Based Strategy Predicts Selective and Synergistic Drug Combinations for Relapsed Acute Myeloid Leukemia
Article Snippet: .. The library preparation, gel beads in emulsion generation, and cDNA amplification were performed according to the manufacturer’s instructions (10x Genomics), aiming for a target cell capture of 4,000 to 6,000 target cell capture per sample. .. Samples were sequenced on an Illumina NovaSeq 6000 system with read lengths of 28 bp (read 1), 10 bp (i7 index), 10 bp (i5 index), and 90 bp (read 2).

Gene Expression:

Article Title: Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer.
Article Snippet: .. The spatial gene expression process, including tissue permeabilization, second strand synthesis and cDNA amplification, was performed according to the manufacturer’s instructions (10X Genomics; #CG000239). cDNA quality was next assessed using Agilent High sensitivity DNA Kit (Agilent, #5067-4626). .. The spatial gene libraries were constructed using Visium Spatial Library Construction Kit (10X Genomics, PN-1000184).

Article Title: E-cadherin inactivation shapes tumor microenvironment specificities in invasive lobular breast cancer.
Article Snippet: .. Spatial gene expression processing, including tissue permeabilization, second-strand synthesis, and cDNA amplification, was then performed according to the manufacturer’s instructions (10X Genomics; #CG000239), following the workflow previously reported in 33. cDNA quality was subsequently assessed using the Agilent High Sensitivity DNA Kit (Agilent, #5067-4626). .. Spatial gene libraries were generated using the Visium Spatial Library Construction Kit (10X Genomics, PN-1000184).

cDNA Synthesis:

Article Title: Automated high-throughput profiling of single-cell total transcriptome with scComplete-seq
Article Snippet: .. After in-droplet cDNA synthesis, the droplets were de-emulsified to recover cDNA, and cDNA amplification was performed according to the 10X Genomics Chromium protocol. ..

Emulsion:

Article Title: A Machine Learning–Based Strategy Predicts Selective and Synergistic Drug Combinations for Relapsed Acute Myeloid Leukemia
Article Snippet: .. The library preparation, gel beads in emulsion generation, and cDNA amplification were performed according to the manufacturer’s instructions (10x Genomics), aiming for a target cell capture of 4,000 to 6,000 target cell capture per sample. .. Samples were sequenced on an Illumina NovaSeq 6000 system with read lengths of 28 bp (read 1), 10 bp (i7 index), 10 bp (i5 index), and 90 bp (read 2).

other:

Article Title: Neoantigen-specific CD8 T cells with high structural avidity preferentially reside in and eliminate tumors.
Article Snippet: 14 PCR cycles were used to amplify cDNA and the final material was divided into two fractions: first fraction was target-enriched for TCRs and V(D)J library was obtained according to manufacturer protocol (10X Genomics).



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